NOT DESCRIBED IN PREVIOUS SECTIONS. 



525 



cloudiness at the end of twenty-four hours ; later the bouillon is clear and a 

 white sediment is seen at the bottom of the tube. In bouillon cultures 

 especially, the bacillus grows out into long filaments. 



Pathogenesis. From 0.25 to 1 cubic centimetre of a bouillon culture 

 injected into the pleural cavity of a rabbit caused a development of all of 

 the symptoms of influenza (Brustseuche) viz. , elevation of temperature at 

 the end of five or six hours, cough, nasal discharge, dyspnoea, and death 

 usually in from three to five days. The autopsy showed a distinct pleuro- 



fneumonia and a general blood infection by the bacillus in question, 

 njections into the circulation also give rise to the symptoms of influenza, 

 including pneumonia, and to death at the end of from ten to fourteen days. 

 Subcutaneous injections resulted in the development of an abscess and of ex- 

 tensive necrosis of the tissues, but did not cause a general blood infection. 

 Guinea-pigs were somewhat less susceptible than rabbits, but injections into 

 the pleural cavity produced similar symptoms and death at a later date. 

 White mice and house mice, as a result of intra peritoneal injections, died 

 within two or three days from general blood infection. 



172. BACILLUS BOVIS MORBIFICANS 



Obtained by Basenau (1893) from the flesh of a cow, which is supposed to 

 have died from puerperal fever and was condemned by the inspector at the 

 slaughter-house in Amsterdam. 



Morphology. Short bacilli, with rounded ends, two to two and one-half 

 times as long as broad, usually united in pairs. 0.3 to 0.4 ^ broad. 



Biological Characters. An aerobic and facultative anaerobic, non- 

 liquefying, actively motile bacillus. Does not stain by Gram's method. 

 Does not form spores is killed in one minute by exposure to a temperature 

 of 70 C. Does not coagulate milk. In media containing glucose causes a 

 moderate development of gas. Grows at a temperature of 9 C., best in 

 incubating oven at 37 C. In bouillon, at 37 C., a uniform clouding of the 

 medium occurs in twenty-four hours ; later, a thin, smooth pellicle forms 

 011 the surface, this is readily broken up by gentle agitation and falls to the 

 bottom, where a grayish- white mass accumulates. 



In gelatin stick cultures a slender, yellowish-white growth is seen along 

 the line of puncture, and a white, thick layer, with more or less irregular 

 outlines, is slowly developed on the surface. In streak cultures the growth is 

 like that of the ' ' colon bacillus. " Upon agar, at 37 C., at the end of twenty- 

 four hours, an abundant grayish-white layer is developed. Upon potato it 

 grows more slowly and forms a soft, yellow layer, which never acquires a 

 brown color. 



Pathogenesis. Causes a fatal infection in mice, white rats, guinea-pigs, 

 rabbits, and calves. Mice and guinea-pigs succumb to subcutaneous injec- 

 tions, rabbits to intra-peritoiieal infection, and calves to intraperitoneal injec- 

 tions, or from the iiigestion of milk containing the bacilli. Young guinea- 

 pigs may be infected through the mother's milk. (This bacillus belongs to 

 the "colon group" and is probably a pathogenic variety of Bacillus coli 

 communis. G. M. S.) 



173. BACILLUS PISCICIDUS (Fischel and Enoch). 



Obtained by Fischel and Enoch (1892) from an infected carp. 

 Morphology. Bacilli solitary or in chains of four to five elements, 

 long- and 



' \JL m V/J.1.CW1.AJ.U \SJL i\_f-t.i \J\S AA T V> V/ L\s JJL J.V^XX lAJj J. ^ 



to 3/* long and 0.25// thick. Stains by the usual aniline colors and by 

 Gram's method. 



Biological Characters. An aerobic and facultative anaerobic, non- 

 motile, liquefying bacillus. Forms spores. In gelatin plates forms round 

 colonies of a pale yellowish-brown color, having a slightly toothed border 

 and a granular surface. At the end of twenty-four hours a narrow zone of 



