528 PATHOGENIC AEROBIC BACILLI 



Biological Characters. Forbes (1891) says: "I have lately succeeded, 

 in conjunction with Professor Burrill, in making pure cultures in consid- 

 erable numbers in both animal and vegetable media." . . . "We have ob- 

 tained successful cultures in all the neutral and alkaline fluids and in none 

 of the*acid ones." Non-motile and does not form spores. 



178. BACILLUS MONACH^E (v. Tubeuf). 



Obtained by v. Tubeuf (1892) from infected caterpillars of Liparis 

 monacha. 



Morphological and Biological Characters. Short, motile, aerobic, non- 

 liquefying bacilli, which grow in the usual culture media at the room tem- 

 perature. 



179. MICROCOCCUS OF BRUCE. 



Obtained by Bruce (1892) from the spleen, post-mortem of cases of so- 

 called "Malta fever." 



Morphology. Micrococci, about .33 p in diameter, solitary or in pairs 

 never in chains. 



Biological Characters. An aerobic, non-liquefying, micrococcus. Does 

 not stain by Gram's method. Grows best in nutrient agar. In stab cultures 

 no growth is seen for several days. "At length the growth appears as 

 pearly- white spots scattered around the point of puncture and minute, round, 

 white colonies are also seen along the course of the needle track " ; these 

 increase in size and after some weeks a rosette-shaped growth is seen upon 

 the surface, and the growth along the line of puncture has a yellowish-brown 

 color. At the end of nine or ten days, at 37 C., some of the colonies on the 

 surface of nutrient agar are as large as No. 4 shot; by transmitted light 

 they have a yellowish color at the centre, and the periphery is bluish-white ; 

 by reflected light they have a milky-white color. At 25 C. colonies first 

 become visible at the end of about seven days, at 37 C. in three to four 

 days. Does not grow upon potato. Very scanty growth upon nutrient 

 gelatin at 22 C. at the end of a month. 



Pathogenesis. Pathogenic for monkeys, which suffer from fever as a 

 result of subcutaneous inoculations and usually (three out of four experi- 

 mented upon) die in from thirteen to twenty-one days. The spleen is found 

 to be enlarged and contains the micrococcus. Not pathogenic for mice, 

 guinea-pigs, or rabbits. 



180. BACILLI OF GUILLEBEAU (a, 6, and C). 



Obtained by Guillebeau from the milk of cows suffering from mastitis, 

 and found by Freudenreich to produce an abnormal fermentation of cheese, 

 characterized by the presence of large cavities ("boursouflement") and by a 

 very bad taste. 



BACILLUS a. 



Morphology. Varies considerably in size, and may resemble a micrococ- 

 cus in form ; usually 1 // broad and 1 to 2 ft- long. 



Stains with the usual aniline colors, but rather feebly ; does not stain by 

 Gram's method. 



Biological Characters. A.naerobics,ndfact<lf<ifir<> anaerobic, slightly 

 motile, non-liquefyingl>a<ci\]us. Spore formation not observed. Grows in 

 the usual culture media at the room temperature. Upon gelatin plates the 

 deep colonies are spherical, granular, and yellowish in color ; upon the sur- 

 face they are round and granular at first, later they become opaque and re- 

 semble a drop of wax. In gelatin stick cultures development occurs all 

 along the line of puncture, and upon the surface a whitish layer is formed. 



