530 PATHOGENIC AEROBIC BACILLI 



culture media at the room temperature still more rapidly at 37 C. The 

 cultures give off a peculiar odor. Forms spores. 



Pathogenesis. According- to Galtier injections into the lungs or into the 

 trachea, in calves, pigs, rabbits, or guinea-pigs, cause a development of the 

 disease, "with a predominance of the pleuro-pulmonary lesions." 



183. BACILLUS PSEUDO-TUBERCULOSIS OF PREISZ. 



Obtained by Preisz (1894) from an infected sheep. 



Morphology. Resembles the bacillus of diphtheria, but is smaller. 



Biological Characters. An aerobic, and facultative anaerobic, non- 

 motile bacillus. Does not |pow in nutrient gelatin at the room temperature. 

 In bouillon a scaly pellicle is formed upon the surface which breaks up upon 

 slight agitation ; the bouillon is but slightly clouded. Upon blood serum 

 the colonies, at 37 C., have a golden or orange-yellow color ; this varies con- 

 siderably in different cultures. Does not form pigment in agar cultures, 

 does not grow upon potato. Stains by Gram's method. 



Pathogenesis. Pure cultures inoculated into rabbits or guinea-pigs give 

 rise to a pseudo-tuberculosis of the lymphatic glands, the spleen, the liver, 

 the mesentery, etc. This ends fatally in from ten to thirty-five days. 



184. BACILLUS PSEUDO-TUBERCULOSIS MURIUM. 



Obtained by Kutscher (1894) from a mouse which died in the laboratory. 



Morphology. Slender bacilli, which frequently have pointed extremities, 

 about the length of the diphtheria bacillus, and like this bacillus quite vari- 

 able in form. 



Biological Characters. An aerobic and facultative anaerobic, non- 

 motile, non-liquefying bacillus. Spore formation not demonstrated. Stains 

 by Gram's method. Upon agar plates small yellowish colonies are devel- 

 oped at the end of twenty-four hours, at 37 C. ; these have a finely granular 

 centre and a dentate margin ; between the sharply dentate processes are seen 

 short, relatively thick projections. The superficial colonies are in the form 

 of delicate, transparent, white layers ; these resemble colonies of Streptococcus 

 pyogenes. They reach the limit of their development in four or five days. 

 Upon gelatin plates similar colonies are developed, which become visible at 

 the end of forty-eight hours and continue to increase in size for twelve to fif- 

 teen days. In bouillon a slight clouding of the culture medium occurs at 

 the end of twenty -four to fortv-eight hours, and later a finely granular de- 

 posit is seen at the bottom of the tube ; upon the surface a thin pellicle is 

 formed, made up of coffin-shaped crystals. In milk the growth is abundant, 

 but does not cause any perceptible change in the culture medium. Upon 

 potato no development occurs. 



Pathogenesis. Pathogenic for mice, in which pseudo-tubercles of the 

 lungs are developed as a result of the subcutaneous injection of a small 

 amount of a pure culture. Injections into the peritoneal cavity are fatal 

 in from three to five days. Not pathogenic for rabbits or guinea-pigs. 



