C YS TOP US CA NDID i '.s. 45 



MINUTE ANATOMY. 



Mount a transverse section of an alcoholic specimen of a 

 stem or leaf bearing Cystopus, and under low power 

 notice 



1. A layer of short vertical filaments, conidiophores," 

 together forming the hymenium, which appear to arise 

 from the tissues of the host and bear on their free 

 extremities 



2. Chains of rounded conidia, now mostly detached. 



The vegetative portion of the plant, consisting of branch- 

 ing filaments pervading the tissues of the host, can rarely 

 be made out even after staining, without specially skillful 

 manipulation. 



3. The everted membrane formed from the surface cells of 

 the host, formerly covering the sorus. 



4. Draw. 



Under high power notice 



5. The conidia : exact shape, wall and contents. 



6. The delicate neck or pedicel supporting each conidium 

 before becoming detached. 



7. Draw a conidiophore with its conidia. 



Take a piece of the host bearing conidia and boil for a 

 minute or two in potassic hydrate ; remove a portion to the 

 slide, tease apart thoroughly with needles, and stain with 

 chlor-iodide of zinc. Notice 



8. Much branched, often matted filaments, mycelium, 

 pulled out from the tissues of the host. 



2 Cf . fig. 8. 



