PIN us s j v i /: s TRIS. 1 3 1 



form conspicuous yellow clusters at the base of the 

 young shoots. The female flowers are quite inconspic- 

 uous, in small oval clusters of a pinkish color, project- 

 ing slightly beyond the ends of the young shoots. 

 The tree bearing abundant male flowers usually bears 

 few female ones, and vice versa. These flowers when 

 collected should be preserved in alcohol. A few weeks 

 later the two-year-old cones, which will be found just 

 below the new shoots, should be collected and pre- 

 served in alcohol. If the plant is to be studied in 

 spring or summer, some of the large terminal buds 

 should be collected in the late autumn, winter or early 

 spring preceding, and preserved in alcohol. Leaves and 

 stems should be gathered about the first of July, and 

 preserved in alcohol. Mature cones should be gathered 

 in winter or early spring and allowed to dry, care being 

 taken to prevent losing the seeds, which will shake out 

 on drying. 



Fresh leaves and stems may be used for the study 

 of the gross anatomy, but if used for the minute 

 anatomy it is well before cutting sections to place them 

 in alcohol for a few days to get rid of the resin which 

 exudes and gums the fingers and knife unpleasantly. 

 Before cutting sections of stems or leaves which have 

 been preserved in alcohol and before dissecting the male 

 and female flower clusters, it is well to place them for 

 a day in a mixture of equal parts of alcohol and 

 glycerine, which renders them somewhat easier to 

 manipulate. They may, however, be used direct from 

 the alcohol. 



The requisites for the complete study are stems, 



