THE FURTHER DEVELOPMENT OF THE EMBRYO. 



145 



The order of appearance of the limbs varies in the different forms. 

 In Pallene, according to Morgan, the first to develop is the most 

 anterior pair; these limbs lie near the mouth and are chelate, but 

 their first appearance has not been observed with certainty. The next 

 pair to arise is the fourth, and, in the gap which naturally occurs 

 between these limbs, two pairs 

 of ganglia are visible, those of 

 the second and third pairs of 

 limbs (Figs. 66 and 67 ^1). 

 The fourth pair of limbs is 

 followed by the fifth and sixth. 

 The third pair develops later, 

 but the second pair is alto- 

 gether wanting in Pallene, and 

 the seventh pair, like the 

 third, appears a short time 

 before the embryo leaves the 

 egg-envelope. Pallene is thus 

 seen to possess, as an embryo, 

 all the limbs of the adult. 

 In most other Pantopoda, 

 however, this is not the case, 

 only three pairs of limbs being 

 usually developed within the 



egg-envelope. Nymphon hrevicaudatum resembles Pallene in possess- 

 ing all the limbs of the adult at the time of hatching (Hoek). 



While the limbs are appearing and gradually developing, the rudi- 

 ment of the nervous system also undergoes further differentiation. 

 Five pairs of large ganglia can be distinctly made out (Fig. 67 A). 

 They belong to the segments carrying the second to the sixth limbs. 

 It would be interesting to discover the relations of the ganglia which 

 innervate the first pair of limbs to the supra-oesophageal ganglion, 

 i.e., whether they represent a post-oral pair of ganglia fused with 

 the supra-oesophageal ganglion. The two anterior of these five pairs 

 of ganglia approximate closely to each other later (Fig. 72 B), and 

 in the adult these two ganglia, belonging to the second and third 

 limbs, are united. The ganglia of the first three pairs of ambulatory 

 limbs (Fig. 67 .4), which appear early, are followed at a much later 

 stage by those of the fourth pair (the seventh pair of limbs) and 

 the abdominal ganglia. 



In each of the ectodermal thickenings which represent the rudi- 



h 



Fig. 66.— Superficial aspect of an egg of Pallene, 

 showing the anterior part of the embryonic 

 rudiment (after Morgan), g, rudiments of the 

 supra-oesophageal ganglia ; g/i-g/y, ventral 

 ganglia belonging to the segment carrying the 

 second, third, and fourth pairs of limbs; m, 

 mouth ; /, first limb ; IV, rudiment of the 

 fourth limb. 



