18 HISTOLOGICAL TECHNIC 



Ammonia or potash alum, 7 . 5 gm. 



Distilled water, 200.0 c.c. 



Boil for 10 minutes to sterilize; cool and add the following 

 solution: 



Haematoxylin crystals, o . i gm. 



Alcohol 95-per-cent., 10. o c.c. 



Four grams chloral hydrate are then added to the mixture 

 to prevent growth of germs. 



This dye may be used in full strength or diluted with alum water 

 It stains in from two to five minutes. 



2. DelafieWs HcBmatoxylin. 



Haematoxylin crystals, i gm. 



Alcohol, 6 c.c. 



Ammonia alum, saturated aqueous solution, 100 c.c. 



The haematoxyhn should be first dissolved in the alcohol and then 

 added to the alum solution. The mixture should next be allowed to 

 stand in the light for from seven to ten days to ripen. It is then 

 filtered, and to the filtrate are added: 



Glycerin, 25 c.c. 



Wood naphtha, 25 c.c. 



The mixture is again allowed to stand for from two to four days and 

 filtered. It may be used full strength or diluted with equal parts of 

 water. It stains in from two to five minutes. 



3. Heidenhain's HcBmatoxylin. 



Ha^matoxylin crystals, i gm. 



Water, 100 c.c. 



Sections are first placed for from four to eight hours in a 2.5-per-cent. 

 aqueous solution of ammonium sulphate of iron. They are then 

 washed in water and transferred to the haematoxylin solution until 

 they are intensely blue or black (usually several hours). The sec- 

 tions are now washed in water and differentiated by again placing 

 in the iron solution till they have a light grayish color. After this 

 they are thoroughly washed in water. 



4. Mayer^s Hamalum. 



Haematein, i gm. 



Alcohol, 50 c.c. 



Ammoiiia alum, 5-per-cent. aqueous solution, 1,000 c.c. 



