GENERAL TECHNIC 19 



The hacmatein is first dissolved in the alcohol, after which the alum 

 is added. This dye does not require any ripening, and is thus avail- 

 able for immediate use. It is a rapid nuclear dye usually requiring 

 not more than from three to five minutes. 



5. A combination of Gage's and Mayer's formuL-c makes a very 

 satisfactory nuclear dye. 



Hajinatein, 5 gm. 

 Alcohol, 50 c.c. 

 Chloral hydrate, 20 gm. 

 Ammonia alum, 5-per-cent. aqueous solution (steril- 

 ized), 1,000 c.c. 



The haimatein is first dissolved in the alcohol and then added with 

 the chloral hydrate to the alum solution. This solution is used in 

 full strength and stains in from three to five minutes. 



6. Weigerfs Hcematoxylin. 



Two stock solutions should be made up as follows: 



A. i-per-cent. haematoxylin, in 96-per-cent. alcohol. 



B. Hydrochloric acid (sp. gr. 1.126), 10 c.c. 

 Ferric chloride, 30-per-cent., 40 c.c. 

 Distilled water, 950 c.c. 



For use, mix equal parts of A and B- The mixture will keep two or 

 three days. This is a rapid stain usually requiring not more than a 

 minute. A more brilliant nuclear stain may be obtained by over- 

 staining and then decolorizing. After the stain wash the sections 

 in water and then decolorize to the proper degree in weakly acidu- 

 lated water. To stop the action of the acid the sections should be 

 dipped in water made slightly alkaline with ammonia. This is an 

 excellent stain and gives brilliant results. It is especially good in 

 cases where the material has become old and lost its afiinity for ordi- 

 nary haematoxylin stains. 

 ih) Alum-carmine. 



Carmine, 2 gm. 



Alum, 5 gm. 



Carbolic acid, 2 gm. 



Water, 100 c.c. 



The alum is first dissolved in the 100 c.c. of warm distilled water, 

 after which the carmine is added. This mixture is then boiled for 

 twenty minutes, allowed to cool, and filtered. The carbolic acid is 

 then added. This is a slow-acting pure nuclear dye. 



