CONTENTS 



PART I.-HISTOLOGICAL TECHNIC 



CHAPTER I 



General Technic. Page 



General Considerations, 3 



Examination of Fresh Tissues, 3 



Dissociation of Tissue Elements, 4 



Teasing, 4 



Maceration, 4 



Preparation of Sections, 5 



Fixation, 5 



Hardening, 9 



Preserving, . . . lo 



Decalcifying, lo 



Embedding, n 



Celloidin Embedding, ii 



Paraffin Embedding, 13 



-Section Cutting, 14 



Celloidin Sections, 15 



Paraffin Sections, 15 



Frozen Sections, 16 



Staining, 17 



Nuclear Dyes, 17 



Plasma Dyes 20 



Staining Sections, 20 



Staining in Bulk, / 22 



Mounting, 22 



Staining and Mounting Paraffin Sections, 24 



Injection, 25 



CHAPTER II 



Special Staining Methods. 



Silver Nitrate Method of Staining the Intercellular Substance, 28 



Chlorid of Gold for Demonstrating Connective-tissue Cells, 28 



Weigert's Elastic Tissue Stain 28 



Verhoeff's Diflerential Elastic Tissue Stain, 28 



Golgi's Chrome-silver for staining Secretory Tubules, 29 



Mallory's Phosphomolybdic Acid Haematoxylin Stain for Connective Tissue, 29 



Mallory's Phosphotungstic Acid Haematoxylin Stain for Connective Tissue, . 30 



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