NERVE TISSUE 149 



studying the internal structure of the nerve cell and processes as demonstrated by 

 the method of Nissl. 



(3) Medullated Nerve Fibres (fresh). — Place a small piece of one of the sciatic 

 or lumbar nerves of a recently killed frog in a drop of salt solution and tease lon- 

 gitudinally. Cover and examine as quickly as possible. Note the diameter of 

 the axone and of the medullary sheath and the appearance of the nodes of Ran- 

 vier. An occasional neurilemma nucleus can be distinguished. 



(4) Medullated nerve fibres — fibres from the cauda equina (this material has 

 the advantage of being comparatively free from fibrous connective tissue) — -are 

 fixed in formalin- IMiiller's lluid (tcchnic 5, p. 7), and hardened in alcohol. Small 

 strands are stained twenty minutes in strong picro-acid-fuchsin solution (technic 

 2, p. 20), washed thoroughly in strong alcohol, cleared in oil of origanum, thor- 

 oughly teased longitudinally and mounted in balsam. 



General References for Further Study of Tissues 



Barker: The Nervous System. 



Bethe: Allgemeine Anatomic und Physiologic des Nervensystem. 

 Cabot: A Guide to the Clinical Examination of the Blood for Diagnostic 

 Purposes. 



Ewing: Clinical Pathology of the Blood. 



Hertwig: Die Zelle und die Gewebe. 



KoUiker: Handbuch der Gewebelehre. 



Prenant, Bouin et Maillard: Traite d'Histologie. 



Ranvier: Traite Technique d'Histologie. 



Van Gehuchten: Le Systeme nerveux de I'homme. 



Wood: Laboratory Guide to Clinical Pathology. 



