338 THE ORGANS 



Nerves. — Sensory medullated iibres pierce the muscularis, branch 

 repeatedly in the stroma, lose their medullary sheaths, and terminate 

 among the cells of the lining epithelium. Sympathetic hbres form 

 plexuses in the fibrous coat, where they are interspersed with numerous 

 small groups of ganglion cells. Axones of these syrApathetic neurones 

 penetrate the muscularis. Here they form plexuses, from which are 

 given off terminals to the individual muscle cells. 



For development of urinary system see page 382. 



TECHNIC 



(i) Fix the simple kidney of a rabbit or guinea-pig in formalin-^Miiller's fluid 

 (technic 6, p. 7). Make sections through the entire organ including the papilla 

 and pelvis, stain with hsematoxylin-eosin (technic i, p. 20), and mount in balsam. 

 This section is for the study of the general topography of the kidney. 



(2) Fix small pieces from the different parts of a human kidney in formalin- 

 Miiller's fluid or in Zenker's fluid. Thin sections should be made, some cutting 

 the tubules longitudinally, others transversely, stained with haematoxylin-eosin 

 and mounted in balsam. 



(3) Blood-vessels. — For the purpose of demonstrating blood-vessels of the 

 kidney the method of double injection is useful (page 27). 



(4) Ureter. — Cut transversely into short segments, fix in formalin- ^Miiller's 

 fluid (technic 6, p. 7), and stain transverse sections with haematoxylin-eosin 

 (technic i, p. 20), or with haematoxylin-picro-acid-fuchsin (technic 3, p. 21). 

 Mount in balsam. 



(5) Bladder (technic i, p. 250, or technic 2, p. 260). By the latter method 

 any desired degree of distention may be obtained. 



General References for Further Study 



KoUiker: Handbuch der Gewebelehre,. vol. iii. 



Gegenbauer: Lehrbuch der Anatomie des Menschen, vol. ii. 



Henle: Handbuch der Anatomie des Menschen, vol. ii. 



Johnston: A Reconstruction of a Glomerulus of the Human Kidney. Johns 

 Hopkins Hosp. BuL, vol. xi., 1900. 



Miiller: Ueber die Ausscheidung des Methylenblau durch die Nieren. 

 Deutsches Archiv f. klin. Med., Bd. 63, 1899. 



