THE REPRODUCTIVE SYSTEM 385 



TECHNIC 



(i) A human uterus — if possible from a young adult — or, if this cannot be ob- 

 tained, the uterus of a cat or dog, is cut transversely into slices about i cm. thick 

 and fixed in Zenker's fluid (technic lo, p. 8) or in formahn-Mullcr's fluid (technic 

 6, p. 7). For topography these slices are cut in half through the middle of the 

 uterine cavity and sections made through the entire half organ. These are stained 

 with haematoxjdin-picro-acid-fuchsin (technic 3, p. 21) and mounted in balsam. 

 For details of the mucous membrane, cut away most of the muscle from around 

 the half slice, being careful not to touch the mucous surface; make thin sections, 

 stain with hajmatoxylin-eosin (technic i, p. 20), and mount in balsam. 



(2) Sections of the cervix may be prepared in the same manner as the 

 preceding. 



(3) Placental tissue may be cut into small cubes and treated with the same 

 technic (i). 



(4) If a human or animal uterus with the placenta m situ is obtainable it 

 should be cut into thin slices and fixed in formalin-Miiller's fluid. The blocks 

 of tissue should be so arranged that sections include the utcro-placental junc- 

 tion. They may be stained with haematoxylin-eosin or with ha^matoxylin-picro- 

 acid-fuchsin (see above). 



(5) Treat pieces of the human vagina according to technic i, p. 259. 



General References for Further Study 



Ballowitz: Weitere Beobachtungen iiber den feineren Bau der Saugethier- 

 Spermatozoen. Zeit. f. wiss. ZooL, Bd., Hi, 1891. 



Hertwig: Lehrbuch der Entwickelungsgeschichte des Menschen und der 

 Wirbelthiere, Jena, 1896. 



Kolliker: Handbuch der Gewebelehre des Menschen. 



Xagel: Das menschliche Ei. Arch. mik. Anat., Bd. xxxi, 1888. 



Ruckert: Zur Eireifung der Copepoden. Anat. Hefte, I. Abth., Bd. iv, 

 1894. 



Schaper: Chapter on the Placenta in Stohr's Text-book of Histology, 5th ed. 



Sobotta: Ueber die Bildung des Corpus luteum bei der Maus. Arch. f. 

 mik. Anat., Bd. xlvii, 1S96. — Ueber die Bildung des Corpus luteum beim 

 Kaninchen. Anat. Hefte, I. Abth., Bd. viii, 1897. 



2J 



