264 PATHOGENIC BACTERIA. 



days upon fresh media. In my experience freshly iso- 

 lated bacilli manifest this capability more markedly than 

 those which have been for some time part of the labo- 

 ratory stock of cultures, and subject to frequent trans- 

 plantation. 1 



Upon potato a luxuriant greenish or brownish, smeary 

 layer is produced. Milk is coagulated and peptonized. 



This bacillus is highly pathogenic for laboratory ani- 

 mals. About 1 c.cm. of a fresh bouillon culture, if in- 

 jected into the subcutaneous tissue of a guinea-pig or a 

 rabbit, causes a rapid edema, a suppurative inflammation, 

 and death in a short time (twenty-four hours). Some- 

 times the animal lives for a week or more, then dies. 

 There is a marked hemorrhagic subcutaneous edema at 

 the seat of inoculation. The bacilli can be found in the 

 blood and in most of the tissues. 



When the dose is too small to prove fatal, suppuration 

 occurs in many cases. 



When sterilized cultures are injected, the same results 

 follow, a relatively larger quantity, of course, being re- 

 quired. 



Intraperitoneal injections cause suppurative peritonitis. 



Blum reports a case of pyocyaneus infection with pyo- 

 cyaneus-endocarditis in a child. 2 



Lartigau, 3 in his study of "The Bacillus Pyocyaneus 

 as a Factor in Human Pathology," speaks as follows: 

 " The Bacillus pyocyaneus, like many pathogenic micro- 

 organisms, is occasionally found in a purely saprophytic 

 role in various situations in the human economy. It 

 has been found in the saliva by Pausini, in sputum by 

 Frisch, and in the sweat by Eberth and Audanard. 

 Abelous demonstrated its presence in the stomach as a 

 saprophyte. Its existence in suppurating wounds has 

 long been known, and Koch early detected its presence 

 in tuberculous cavities, regarding it as an organism in- 



1 See Centralbl.f. Bakt., xxi., April 6, 1897, p. 473. 



2 Centralbl.f. Bakt. u. Parasitenk., Feb. 10, 1899, xxv., No. 4. 

 8 Phila. Med. Journal, Sept. 17, 1898. 



