CEREBROSPINAL MENINGITIS. 277 



able quantity of the fluid (say about 1 c.ctn.) must be 

 used. 



In the 55 cases studied by Councilman, Mallory, and 

 Wright the cocci were found by culture or by micro- 

 scopic examination in 38. 



The cocci have also been cultivated from the nasal 

 discharges in the 6 cases studied by Weichselbauin and 

 in 18 studied by Scherer. 



The results of animal inoculations made with the 

 Diplococcus intracellularis meningitidis are on the whole 

 disappointing. Subcutaneous inoculations into the lower 

 animals are continually without effect. Intrapleural and 

 intraperitoneal injections of cultures of the organism into 

 mice and guinea pigs are sometimes fatal, the dead animals 

 showing a serofibrinous inflammation with the presence of 

 the cocci. The intravenous injection of the coccus into rab- 

 bits is followed by death without important or conclusive 

 symptoms, and usually without the cocci in the blood. 



Weichselbauin endeavored to reproduce the original 

 cerebrospinal meningitis in animals by trephining and 

 injecting the cocci beneath the dura. In this manner he 

 inoculated three rabbits and three dogs. Two of the 

 rabbit injections failed, probably because the injected 

 material escaped at once from the wound. The third 

 rabbit died, and showed marked congestion of the mem- 

 branes of the brain and a minute softened and hemor- 

 rhagic area. In these the cocci were found by culture to 

 be abundant. The three dogs all died with congestion 

 and pus formation in the membranes and areas of soften- 

 ing in the brain substance. The cocci were recovered 

 from two of the dogs, but the lesions of the third animal, 

 which lived twelve days, contained none. 



It is not known by what channels the infection by 

 the Diplococcus intracellularis meningitidis takes place. 

 Weichselbaum supposed it might enter by the nasal, 

 auditory, or other passages, especially the nose, where he 

 constantly found it. In this connection it is interesting 

 to note that the only two of the fifty supposedly healthy 



