TETANUS. 383 



growth in agar-agar is very luxuriant and is accompanied 

 by the evolution of much gas. Upon obliquely solidified 

 agar-agar the colonies are round, circumscribed, and often 

 encompassed by a narrow clear zone, which is often notched. 

 The organism grows in serum only in vacuum. The 

 spores are killed at 8o° C. 



The organism produced no symptoms in mice, guinea- 

 pigs, and rabbits even when 2-5 c.cm. were subcutane- 

 ously introduced. 



Sanfelice 1 and Lubinski 2 have also observed in earth 

 and meat infusions a bacillus which is morphologically 

 and culturally like the tetanus bacillus, but differs from 

 it in not producing any toxin. 



Kruse 3 has also described a tetanus-bacillus-like micro- 

 organism that grows aerobically. It is not pathogenic. 



1 Zeitschrift filr Hygiene, vol. 14. 



1 Centralbl. f. Bakt. u. Parasitenk., xvi., 19. 



* Haggis, Die Mikroorganismen, vol. ii., p. 267. 



