TYPHOID FEVER. 473 



80 grams of gelatin, 5 grains of Liebig's beef-extract, 5 

 grams of sodium chlorid, and 10 grams of glucose to the 

 liter. The agar is dissolved in the 1000 c.cm. of water, to 

 which have been added the beef-extract and sodium chlorid. 

 When the agar is completely melted the gelatin is added 

 and thoroughly dissolved by a few minutes' boiling. The 

 medium is then titrated to determine its reaction, phenol- 

 phthalein being used as the indicator, and enough HC1 or 

 NaOH added to bring it to the desired reaction — i. e. a re- 

 action indicating 1.5 per cent, of normal acid. To the clear 

 medium add one or two eggs, well beaten in 25 c.cm. of 

 water; boil for forty-five minutes, and filter through a 

 thin filter of absorbent cotton. Add the glucose after 

 cleaning. 



The medium is used in tubes, in which it is planted by 

 the ordinary puncture of the many allied forms studied. 

 The typhoid bacillus alone has the power of cloud- 

 ing this medium uniformly without showing streaks or 

 gas-bubbles. 



The second medium is used for plating. It contains 

 10 grams of agar, 25 grams of gelatin, 5 grams of beef- 

 extract, 5 grams of sodium chlorid, and 10 grams of glu- 

 cose. The method of preparation is the same as for the 

 tube-medium, care always being taken to add the gela- 

 tin after the agar is thoroughly melted, so as not to alter 

 this ingredient by prolonged exposure to high tempera- 

 ture. This preparation should never contain less than 2 

 per cent, of normal acid. Of all the organisms with 

 which Hiss experimented, the Bacillus typhosus alone 

 displayed the power of producing thread-forming colonies 

 upon this medium. 



The colonies of the typhoid bacillus when deep in the 

 medium appear small, generally spherical, with a rough, 

 irregular outline, and by transmitted light are of a vitreous 

 greenish or yellowish-green color. The most character- 

 istic feature consists of well-defined filamentous out- 

 growths, ranging from a single thread to a complete 

 fringe about the colony. The young colonies are, at 



