570 PA THOGENIC BA CTERIA . 



A. Catanni, Jr. 1 trephined rabbits and injected influ- 

 enza toxin into their brains, at the same time trephining 

 control-animals, into some of whose brains he injected 

 water. The results were that animals thus receiving' 

 0.5-1 mgr. of the living culture constantly died in 

 twenty-four hours with all the nervous symptoms of the 

 disease, dyspnea, paralysis beginning in the posterior 

 extremities and extending over the whole body, clonic 

 convulsions, stiffness- of the neck, etc. Control-animals 

 injected with a variety of pathogenic bacteria in the 

 same manner never manifested similar symptoms. The 

 virulence of the bacillus was also observed to increase 

 rapidly when transplanted from brain to brain. 



Wynekoop 2 has successfully employed, for diagnosti- 

 cating influenza and isolating the bacillus, a culture- 

 outfit similar to that used for diphtheria-diagnosis, except 

 that the serum contains more hemoglobin. The swab is 

 used to secure secretions from the pharynx and tonsils, 

 and from the bronchial secretions of patients with in- 

 fluenza, then rubbed over the blood-serum. In many 

 such cultures the minute colonies corresponding to those 

 of the influenza bacillus were found. Those most 

 isolated were picked up with a wire and transplanted to 

 bouillon, from which fresh blood-serum was inoculated 

 and pure cultures secured. 



Carbol-fuchsin was found most useful for staining the 

 bacilli. An interesting observation made by Wynekoop 

 was that influenza and diphtheria bacilli sometimes co- 

 exist in the throat, and that influenza bacilli are present 

 in the sore-eyes of those in the midst of household epi- 

 demics of influenza. 



1 Zeilschrift filr Hygiene, etc., 1896, Bd. xxiii. 



2 Bureau and Division Reports, Department of Health, City of Chicago, 

 Jan., 1899. 



