600 PATHOGENIC BACTERIA. 



a line of finely granular, non-liquefying'colonies. Upon 

 the surface of the gelatin the growth expands so as to 

 form the so-called u nail-growth. " 



The colonies upon gelatin have an irregularly circular 

 form, appear white or straw-yellow by reflected light and 

 olive-green by transmitted light, and are granular. They 

 do not liquefy and do not grow to large colonies. 



In bouillon after twenty-four hours there was a faint 

 clouding of the liquid and subsequently a sedimentation 

 of the bacteria in small clusters. After a week or so 

 the surface of the medium is covered with a delicate 

 pellicle, which grows thicker with the passage of time. 



The bacillus grows quite well anaerobically. It is 

 motile. 



The bacillus is pathogenic for mice, but does not pro- 

 duce characteristic symptoms in any of the experiment- 

 animals. 



In discussing the results of Koplik's work, and com- 

 paring it with their own, which very shortly preceded it, 

 Czaplewski and Hensel suggest that the bacillus is better 

 described as a bacterium than as a bacillus. The finely 

 granular ("fein punktiertes ") appearance described by 

 Koplik, in their observations seems to consist of a deeper 

 staining at the poles of the cells. The growths on 

 gelatin and on Lofner's blood-serum mixture correspond 

 m every way. The agar-agar growths are similar, 

 though a slight difference in color is noted, and is attrib- 

 uted to a difference in the quality of the medium used. 

 The bouillon culture differs, the description of Czaplewski 

 and Hensel being as follows: at the end of a day at 37 ° 

 C. the bouillon is scarcely clouded. At the bottom of 

 the tube is a sharply defined, lentil-like sediment, which 

 arises in the form of slimy threads when the fluid is 

 whirled about, and mixes with the fluid when ener- 

 getically shaken. Neither bacillus grows on potato. 

 Koplik's bacillus was also peculiar in that it was motile. 

 Regarding Koplik's bacillus as identical with their own, 

 Czaplewski and Hensel do not agree with him in believ- 



