102 



APPLIED IMMUNOLOGY 



brought in relation with syphihtic serum, but as yet we 

 are in the dark in regard to the true nature of the 

 phenomenon. 



Several factors other than the antigen are impor- 

 tant in obtaining reliable results with the Wassermann 

 reaction; the most essential of which is the titration 

 and use of minimal amounts of complement. 



Technic of the Wassermann Reaction 



The following is a list of the apparatus, animals, 

 etc., required, and has been made as complete as 

 possible : 



Electric centrifuge. 

 Several 15 c.c. graduated centri- 

 fuge tubes. 

 Several 1 c.c. pipettes, graduated 



in tenths of a c.c. 

 Several 1.2 c.c. pipettes, graduated 



in hundredths of a c.c. 

 Two 10 c.c. pipettes, graduated in 



tenths of a c.c. 

 Capillary pipettes, prepared by 



drawing out 3/8 in. glass tubing. 

 Rubber nipples for capillary 



pipettes. 

 100 1 c.c. glass ampoules. 

 100 small test-tubes, about 5 c.c. 



capacity. 

 Several flasks, capacity 500 c.c. 

 Two flasks, 100 c.c. capacity. 

 Two graduated 100 c.c. cylinders. 

 Several Petri dishes. 



Normal salt solution (0.85 per 

 cent.). 



Sodium citrate solution (1 per 

 cent, in normal salt solution). 



Wire racks for holding small test- 

 tubes. 



Platinum loop with glass handle. 



Scissors, scalpel, dissecting forceps. 



2 c.c. all glass hypodermic syringe 

 with small needle. 



Wax pencil. 



95 per cent, alcohol, ether, xylol, 

 glycerin. 



Water-bath. 



Centigrade thermometer. 



Incubator. 



Bunsen burner. 



Refrigerator. 



Sheep, rabbits, guinea-pigs. 



All glass-ware and solutions mentioned above 

 must be sterilized before using. 



