FIXATION OF COMPLEMENT 



107 



xylol, and the suspension slowly injected into it 

 through the skin in the direction of the circulation 

 (Fig. 8). Usually, to obtain a sufficiently powerful 

 h^emolytic amboceptor, three injections at intervals of 

 3 or 4 days are required. The second injection should 



Fig. 8. — Showing method of intravenous injection or immunization of rabbit with 

 10 per cent, suspension of sheep's blood-corpuaclea for the production of haemolytio 

 amboceptor. 



consist of 1.5 c.c. and the third of 2 c.c. of the sus- 

 pension. Three or 4 days after the third injection, 

 about 2 c.c. of the rabbit's blood is drawn from the ear 

 by puncturing a vein, and the serum tested for its 

 hsemolytic power against sheep's corpuscles. Thus in 



