186 APPLIED IMMUNOLOGY 



redness of the caruncle, sometimes involving the en- 

 tire palpebral conjunctiva and lids. 



Vaughan, Jr., states that he has never seen any- 

 ill effects as a result of the application of the test. His 

 technic consists in dissolving a tuberculin tablet in five 

 minims of boiling hot water, producing a one per cent, 

 solution. One drop of this, after cooling, is instilled 

 into the conjunctival sac; if no reaction takes place, 

 at the end of a week a second instillation of one drop 

 of one-half per cent, solution is instilled into the same 

 eye. He believes the zymogen created by the first in- 

 stillation is activated by the second, causing the anti- 

 tuberculin ferment to attack the tuberculin, thereby 

 liberating the toxic cleavage product in large amount, 

 even though the second dose is but one-half of the 

 first. As soon as a reaction becomes distinctive, the 

 conjunctiva is copiously flushed with a solution of 

 boric acid, in order to prevent undue inflammation. 

 This tuberculin test has been unsuccessfully applied to 

 the nasal and vaginal mucosa. 



JLuetin Cutaneous Reaction in Syphilis, — Noguchi, 

 in 1911, described an allergic cutaneous reaction in 

 syphilis. Following the successful cultivation of the 

 Treponema pallidum {S pirochceta pallida) in ascitic 

 fluid and ascitic fluid agar containing pieces of pla- 

 centa, the agar growth was ground to a paste in a 

 mortar, then diluted with the fluid culture, steriHzed 



