232 



APPLIED IMMUNOLOGY 



great concentration. The best strength would ap- 

 pear to be that rendering a phagocytosis averag- 

 ing about four bacteria pro leucocyte. Two opsoniz- 

 ing capillary pipettes, P (patient) and C (control), 

 are prepared so that the lumina are about the diame- 

 ter of a large sized hat-pin. A distance of one-half 



Fig. 27. — Washing the culture of the given bacterium from the culture medium, using 

 the platinum loop, in order to obtain the bacterial suspension. 



or three-quarters of an inch is marked off on the ends 

 of these two pipettes (Fig. 28, 1 and II) . By the use 

 of a small rubber bulb, equal quantities of patient's 

 serum, bacterial suspension and washed leucocytes, 

 permitting a small bubble of air to separate each, are 

 drawn up and mixed by skilful thumb and finger 

 gymnastics, in the pipette designated P. In the same 



