BACTERIAL INOCULATION 289 



among the inoculated group was only 0.123 per cent, 

 as compared with 11.1 per cent, among the uninocu- 

 lated. For protective inoculation three doses of 250, 

 500 and 1000 million, from a week to ten days apart, 

 should be administered. 



Hay Fever, — An allied treatment by antigenic 

 inoculations is that of hay fever by injections of the 

 extracted pollen of rag-weed, on the assumption that 

 this affection is a pollen toxicosis and not an anaphy- 

 lactive expression, a thought that has gained credence 

 recently. Noon and Freeman gauged dosage by the 

 conjunctival reaction and injected their patients at in- 

 tervals of three to ten days with units representing the 

 amount of extract from 0.001 milligramme of pollen. 

 Clowes was able to raise the resistance a thousandfold, 

 although it persisted but five months, by inoculations 

 of one cubic centimetre of 1 : 5,000,000 to 1 : 500,000 

 suspensions. 



Recently, Lowdermilk reported astonishing re- 

 sults with pollen toxin, prepared as follows: One 

 gramme of mixed pollen (250 milligrammes Ambrosia 

 artemisicefolia, 250 milligrammes Ambrosia tiifida 

 and 500 milligrammes of various varieties of soli- 

 daco) was mixed with ten grammes of sterile sea- 

 shore sand in a mortar, moistened with a part of 

 a solution of 100 c.c. physiological saline contain- 

 ing 0.5 per cent, phenol and ground for several 



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