290 APPLIED IMMUNOLOGY 



hours, slowly adding the remainder of the saline- 

 phenol solution until the sand was reduced to an im- 

 palpable powder. The whole was transferred to a 

 sterile flask and allowed to stand with frequent shak- 

 ing, at room temperature, for twenty-four hours. 

 After decantation and centrifugation the toxin was 

 sealed in glass ampoules, containing one cubic centi- 

 metre each. This product was standardized so that the 

 unit represented the quantity of toxin extracted from 

 0.000,001 gramme of pollen. Thus each cubic centi- 

 metre contained ten thousand units. Six to eight 

 inoculations were administered at intervals of one to 

 ten days apart, representing from 25 to 1000 units 

 each. The typical reactionary phenomena simulate 

 the symptoms of an acute attack of hay fever. It is 

 wise to reinforce the pollen toxin injections with inocu- 

 lations of autogenous bacterins prepared from the 

 complicating infection. Such treatment has been ap- 

 plied therapeutically as well as prophylactically. It 

 is, however, of such recent date that no definite con- 

 clusions should be drawn. It may share the same 

 fate as Dunbar's " pollantin " used to produce passive 

 immunity in pollenosis. 



Diseases of Lungs 



Bronchitis. — The following bacteria may be identi- 

 fied: M, catarrhalis, M, paratetragenus, B, influenzcB, 

 Streptococcus, Staphylococcus, B. typhosus, B, coli, Z),. 



