104 



EMBRYOLOGY 



at the posterior margin of the germ-disc of Reptiles and Birds, when 

 their gastrulation begins. An invagination proceeding from this 



point, such as DUVAL has 

 established for the Chick, 

 is unfortunately not as 

 yet proven with sufficient 

 certainty in the case 

 of Mammals ; the origin 

 of the two-layered stage 

 is also still involved in 

 obscurity. 



However, there are in 

 the literature some observa- 

 tions, which, fragmentary 

 as they are, appear to me 

 to be worthy of special 

 regard. 



At the stage at which 

 the blastula has become 

 for a certain distance two- 

 layered (fig. 62), there 1m.- 

 been discovered by HEAPE 

 in the case of the Mole, by 

 SELENKA in the Opossum, 

 and by KEIBEL in the 

 Babbit, at one place of 

 the embryonic spot (pro- 

 bably in the region just 

 described as terminal ridge), 

 a small opening (fig. 64 u) r 

 tvhic/i is possibly to be in- 

 terpreted as blastopore and 

 to be compared with tJie 

 crescentic groove of Birds. 

 Here the two primary <jeru>- 

 layers are continuous with 

 each other, and from here, as well as from the primitive streak, the 

 middle genn-lij<'r takes its origin. I assume that, beginning at 

 this place, the loiver germ-layer has in a still earlier stage been 

 developed by an infolding of a small territory of the single-layered 

 blastula (lig. 59). 



Fig. 62. Blastula of the Rabbit 7 days old without the 

 outer egg- membranes. Length 4*4 mm. After 

 KOLLIKER. Magnified 10 diameters. 



Seen in . ( from above, in B from the side. 



ag, Embryonic spot (area embryonalis) ; rie, the line 

 up to which the blastula is two-layered. 



