II. 



CONDITIONS OF GROWTH. 



BACTERIA only grow in presence of moisture, under certain condi- 

 tions of temperature, and when supplied with suitable pabulum. As 

 they 'do not contain chlorophyll, they cannot assimilate carbon diox- 

 ide, and light is not favorable to their development. 



The aerobic species obtain oxygen from the air and cannot grow 

 unless supplied with it. The anaerobic species, on the other hand, 

 will not grow in the presence of oxygen, and must obtain this ele- 

 ment, as they do carbon and nitrogen, from the organic material 

 which serves them as food. 



As a class the bacteria are supplied with nutriment by the higher 

 plants and animals, the dead tissues of which they appropriate, and 

 which it is their function to decompose, releasing the organic ele- 

 ments as simple compounds which may again be assimilated by the 

 chlorophyll-producing plants. 



Water is essential for the development of bacteria, and many spe- 

 cies have their normal habitat in the waters of the ocean, of lajses, 

 and of running streams ; others thrive upon damp surfaces or in the 

 interior of moist masses of organic material. Many species grow in- 

 differently either in salt or fresh water, but it is probable that cer- 

 tain species will be found peculiar to the waters of the ocean. Some 

 of the water bacteria multiply in the presence of an exceedingly 

 minute amount of organic pabulum, or even in distilled water. This 

 is shown by the experiments of Bolton and others. The author 

 named tested two species of water bacteria (Micrococcus aquatilis 

 and Bacillus erythrosporus) in the following manner : Ten cubic 

 centimetres of distilled water in a test tube were infected with a small 

 quantity of a culture of one of these microorganisms. A drop from 

 this tube was transferred' to the same quantity of distilled water in 

 a second tube, and from this to a third. The number of bacteria in 

 this tube No. 3 was now ascertained by counting, and it was put 

 aside for two or three days, at the end of which time the number was 

 again estimated by counting. In every case there was an enormous 

 increase in the number of bacteria. In order to be sure that the dis- 



