330 PROTECTIVE INOCULATIONS. 



by the same rabid dog ; and the mother of Meister, who had not been 

 bitten. The child had been thrown down by the dog and bitten upon 

 the hand, the legs, and the thighs, in all in fourteen different places. 

 Pasteur commenced the treatment on July 6th, by injecting beneath 

 the skin of this child an emulsion of cord which had been kept for 

 fourteen days; this was followed by twelve more inoculations made 

 on successive days with cord of increasing degrees of virulence the 

 last with cord a day old. On March 1st, 1886, Pasteur reported to 

 the Academy of Sciences the fact that the boy Meister remained in 

 good health and gave detailed information with reference to a number 

 of cases which had since been treated by the same method. 



With reference to the duration of the immunity resulting from 

 these inoculations Pasteur says (1886) that out of fourteen dogs in- 

 oculated with "ordinary street virus," by trephining, at the expiration 

 of a year after the protective inoculations had been practised, eleven 

 resisted ; out of six tested in the same way at the end of two years 

 two proved to be immune. 



In November, 1886, Pasteur communicated to the Academy of 

 Sciences the results of his experiments with reference to a modification 

 of his method as at first employed the so-called intensive method. 

 This modification consisted in making the inoculations with cords of 

 increasing virulence in more rapid succession. 



The method followed at Odessa, as reported by Gamaleia (1887), 

 is shown below, the day being given above and age of the cord 

 below. 



1 2 _3^ 45 6 7 8910 



14-13 12-11 10-9 8-7 6-5 4-3 2-10 8-642 



Since the adoption of this method and the use of larger quantities 

 of virus, according to Gamaleia, there have been no deaths among 

 those inoculated, numbering more than two hundred at the time the 

 report was made. The author last referred to concludes from his ex- 

 perience that " the mortality diminishes in direct relation to the quan- 

 tity of the vaccine injected." 



Bujwid (1889) reports a total of 670 inoculations, with 9 deaths, 

 made at Varsovie during the years 1886, 1887, and 1888. His method 

 is shown below. 



1 2 34567 



12-10 8-643643' 



The results of inoculations made at the Pasteur Institute in Paris 

 during the years 1886 to 1890 are given in the following table : 



