PROTECTIVE INOCULATIONS. 365 



favorable as regards au immunizing or curative effect from inocula- 

 tions of tuberculin in rabbits. Donitz, on the contrary, arrives at the 

 conclusion that when early treatment is instituted iris tuberculosis 

 may be arrested and cured, and the more recent experiments of Tru- 

 cleau (1893) give support to this conclusion. Baumgarten, however, 

 insists that the tuberculin treatment does not prevent metastasis to 

 the lungs after inoculations in the anterior chamber of the eye. 



Pfuhl (1891) treated forty-seven infected guinea-pigs, and at the 

 date of his report forty-four had died tuberculous, but the date of 

 death was somewhat postponed by the treatment. The animals not 

 treated succumbed at the end of eight weeks (average of all controls), 

 and those treated with small doses of tuberculin lived, on the average, 

 ten weeks. With larger doses still more favorable results were ob- 

 tained four lived on an average twelve weeks, and three were still 

 living, eleven, fifteen, and sixteen weeks after infection, at the date of 

 publication. 



Kitasato (1892) also obtained favorable results in the treatment 

 of infected guinea-pigs, and arrives at the conclusion that guinea-pigs 

 which have been cured by the treatment are not susceptible to a sec- 

 ond infection, for a certain time at least. 



Bujwid (1892), in experiments upon guinea-pigs, found that in- 

 fected animals which received from 0.05 to 0.1 gramme of tuberculin 

 within three hours showed an elevation of temperature of 1.5 to 2 C. 

 Thirteen infected guinea-pigs treated with tuberculin lived from two 

 and a half to eight months, while all of the control animals (eighteen) 

 died in from six to nine weeks. The animal which survived eight 

 months was found not to be tuberculous, but presented evidence of re- 

 covery from a former tuberculous process. In two rabbits inoculated 

 in the anterior chamber the iris tuberculosis was favorably influenced 

 by the tuberculin treatment, but general infection occurred, and the 

 animals died about the same time as the controls. Three apes were 

 treated without any apparent result; they all died within two months 

 after infection. 



The experiments of Gramatschikoff, Czaplewski, and Roloff, and 

 of Yamagiva, published in 1892, show that the tuberculin treatment 

 does not cure tuberculous infection in inoculated guinea-pigs and rab- 

 bits, and that the bacilli retain their vitality in such animals in spite 

 of the most persistent treatment. 



Hericourt and Eichet (1892), in experiments made for the purpose 

 of immunizing animals against tuberculous infection, failed to obtain 

 positive results in the most susceptible species guinea-pigs, rabbits, 

 and apes but claim to have succeeded in immunizing dogs by intra- 



