BACTERIA IN CROUPOUS PNEUMONIA. 405 



one part of ninety-five-per-ceiit alcohol to three parts of virus), and that 

 both have a restraining- influence upon the development of this microorgan- 

 ism in culture fluids." 



The following results were obtained by the writer in his experi- 

 ments (1881 and 1883) to determine the germicidal and antiseptic 

 value of the agents named, as tested upon this micrococcus. 



Alcohol. A twenty-four-per-cent solution was effective upon 

 bouillon cultures in two hours. 



Boric Acid. A saturated solution failed to destroy vitality after 

 two hours' exposure, but 1 : 400 restrained development. 



Carbolic Acid. A one-per-cent solution destroys vitality in two 

 hours, and 1 : 500 restrains development. 



Cupric Sulphate destroys the virulence of the coccus in the 

 blood of a rabbit in the proportion of 1 : 400 in half an hour. 



Ferric Sulphate failed to destroy vitality in a saturated solution, 

 but restrained development in the proportion of 1 : 200. 



Hydrochloric Acid destroys the virulence of the blood of a rab- 

 bit containing this micrococcus in the proportion of 1 : 200. 



Iodine, in aqueous solution with potassium iodide, destroys vital- 

 ity in the proportion of 1: 1,000 and prevents development in 1: 4,000. 



Mercuric Chloride. One part in forty thousand prevents the 

 development of this micrococcus, and 1 : 20,000 was found to destroy 

 vitality in two hours. 



Nitric Acid. One part in four hundred destroyed the virulence 

 of rabbit's blood containing this micrococcus. 



Caustic Potash. A two-per-cent solution destroyed vitality in 

 two hours. 



Potassium Permanganate. A two-per-cent solution destroyed 

 the virulence of rabbit's blood containing this coccus. 



Salicylic Acid, dissolved by the addition of sodium biborate. 

 A solution of 1 : 400 prevented development. 



Sulphuric Acid. One part in two hundred destroys vitality, and 

 1 : 800 prevents development. 



In a paper by Bordoni-Uffreduzzi relating to the resisting power 

 of pneumonic virus for desiccation and light, the following results are 

 given : Pneumonic sputum attached to cloths, when dried in the air 

 and exposed to diffuse daylight, retained its virulence, as shown by 

 injection in rabbits, for a period of nineteen days in one series of ex- 

 periments and for fifty-five days in another. Exposed to direct sun- 

 light the same material retained its virulence after twelve hours' 

 exposure. Cultures have far less resistance, and the protection 

 afforded by the dried albuminous material in which the micrococci 

 were embedded, in the experiments referred to, probably accounts 

 for the virulence being retained so long a time. 



