584 



PATHOGENIC ANAEROBIC BACILLI. 



into the abdominal cavity ; or, better still, by the injection of fil- 

 tered serum from animals which have recently succumbed to an ex- 

 perimental inoculation (one cubic centimetre repeated daily for seven 

 or eight days). 



BACILLUS CADAVERIS. 



Obtained by the writer (1889) from pieces of liver and kidney, from yel- 

 low-fever cadavers, which had been preserved for forty-eight hours in an 

 antiseptic wrapping, at the summer temperature of Havana; also in two 



Fio. 165. Bacillus cadaveris; smear preparation from liver of yellow-fever cadaver, kept 

 twenty-four hours in an antiseptic wrapping. X 1,000. From a photomicrograph. (Sternberg.) 



cases from pieces of yellow-fever liver immediately after the autopsy ; also 

 from liver preserved in an antiseptic wrapping from comparative autopsies 

 made in Baltimore. 



Morphology. Large bacilli with 

 square or slightly rounded corners, 

 from 1.5 to 4 ^ in length and about 

 1.2 // broad; frequently associated in 

 pairs; may grow out into straight or 

 slightly curved filaments of from 5 

 to 15 fj. in length. 



Biological Characters. An an- 

 aerobic, non-motile bacillus ; not 

 cultivated in nutrient gelatin ; not 

 observed to form spores. 



Bacillus cadaveris is a strict anae- 

 robic and is difficult to cultivate. I 

 have succeeded best with nutrient 

 agar containing five per cent of 

 glycerin, removing the oxygen 

 thoroughly by passing a stream of 

 hydrogen through the liquefied me- 

 dium. The colonies in a glycerin- 

 agar roll tube (containing hydrogen 



FIG. 166. Bacillus cadaveris, from an anae- 

 robic culture in glycerin-agar. X 1,000. From 

 a photomicrograph. (Sternberg.) 



and hermetically sealed) are opaque, 

 irregular in outline, granular, and of 



