MICROSCOPIC STUDY AND STAINING 115 



In addition to the 5 per cent aqueous solutions of the saturated 

 alcoholic solutions of the dyes mentioned above, a few extremely 

 useful stains for routine work are as follows : 



Loeffler's A Ikalin Methylene-Blue : 



Saturated alcoholic methylene-blue 30 c.c. 



1 to 10,000 Potassium hydrate in water 100 c.c. 



Carbol-Fuchsin': 5 



Basic fuchsin 1 gram 



Alcohol, absolute 10 c.c. 



5% Aqueous carbolic acid 90 c.c. 



To make up this stain mix 90 c.e. of 5 per cent aqueous solution of 

 carbolic acid with 10 c.c. saturated alcoholic basic fuchsin. 

 It can also be made up by weighing out : 



Basic fuchsin 1 gram 



Carbolic acid 5 grams 



Dissolve in distilled water, 90 c.c. filter and add absolute alcohol, 10 c.c. 

 Toluidin-Blue Solution. A very useful stain for general bacteriological 

 work and for diphtheria bacilli. 



Toluidin blue 0.25 gram . 



Acetic acid 2.0 c.c. 



Absolute alcohol , 5.0 c.c. 



Distilled water 100.0 c.c. 



This can be used alone when it gives a stain like Loeffler's methylene blue, 

 but more clear, or else a counterstain of Bismarck brown can be used. 

 Pappenheim-Saathof Methylgreen: 



Methylgreen 0.15 grams 



Pyronin 0.5 grams 



95% Alcohol 5.0 c.c. 



Glycerin 20.0 c.c. 



2% Carbolic acid in water up to 100.0 c.c. 



Stain 1 to 2 minutes, wash, blot. This is a splendid method of staining 

 bacteria in general and it is particularly useful for the staining of phagocytes 

 containing bacteria, as in gonococcus smears and in opsonic work. 



s Saathof, Deut. med. woch., 1905. 



