132 BIOLOGY AND TECHNIQUE 



has been added a few drops of a 10 per cent solution of colophonium in 

 absolute alcohol, keeping the section in constant motion, so that the decoloriza- 

 tion shall be uniform. Control the result under the microscope. When the 

 pink color has returned to the section and the nuclei are still a deep blue, 

 finish the dehydration quickly with absolute alcohol. 



6. Xylol. 



7. Xylol balsam. 



METHOD OF STAINING FOR TUBERCLE BACILLI IN SECTIONS.*" Paraffin 

 Sections. Stain in carbol-fuchsin solution hot for five minutes (or better 

 cold, for twenty-four hours). 



Wash in water. 



Decolorize and countorstain in Gabbet's methylene-blue sulphuric acid 

 mixture for one minute. 



Wash in water. 



Dehydrate in absolute alcohol. 



Clear in xylol. 



Mount in balsam. 



Celloidin Sections. 41 Stain lightly in alum hematoxylin. 



Wash in water. 



Dehydrate in ninety-five per cent alcohol. 



Attach the slide by ether vapor. 



Stain with steaming carbol-fuchsin two to five minutes. 



Wash in water. 



Wash with Orth's acid alcohol (alcohol ninety per cent., 99 c.c.; cone. 

 HC1, 1 c.c.) one-half to one minute. 



Wash in water several changes. 



Treat with ninety-five per cent alcohol until red color is entirely gone. 



Blot and cover with xylol until clear. Mount in balsam. 



METHOD OF STAINING ACTINOMYCES IN SECTIONS. Mallory's Method. 412 

 1. Stain deeply in saturated aqueous eosin ten minutes. 



2. Wash in water. 



3. Anilin gentian-violet two to five minutes. 



4. Wash in normal saline solution. 



5. Weigert's iodin solution (iodin 1, KI 2, and water 100 parts) one 

 minute. 



6. Wash in water and blot. 



7. Clear in anilin oil. 



8. Xylol several changes. 



9. Mount in balsam. 



^Mallory and Wright, 



41 After Mallory and Wright. 



"Mallory and Wright, "Pathol. Tech.," 1904. 



