908 



DISEASES CAUSED BY F1LTHABLE VIRUS 



into the bottom of which pieces of potassium hydrate have been 

 placed. The bottle is then set away in a dark room or closet, the 

 temperature of which is regulated so as to vary little above 25 C. 

 Bacteriological controls as to the sterility of the cord should also 

 be made. 



After drying, pieces of the cord are prepared for injection. This 

 is done in various ways. At the New York Department of Health 1 cm. 

 of the cord is emulsified in 3 c.c. of sterile salt solution, the dose for 

 injection being usually 2.5 c.c. Marx 31 emulsifies 1 cm. of the cord in 

 5 c.c. of sterile bouillon or salt solution, using 1 to 3 c.c. of this for 

 injection according to the age of the cord. For shipment 20 per cent 

 of glycerin and 0.5 per cent of carbolic acid arc added. 



The scheme of treatment is also subject to variations according 

 to the individual customs of various laboratories. The following 

 scheme is the routine of the Pasteur Institute in Paris, as quoted 

 in Kraus and Levaditi, ' ' Handbuch f iir Immunitatsf orschung, ' ' Vol. 

 I, p. 713. 



31 Marx, Deut. med. Woch., 1899, 1900. 



