1094 



PATHOGENIC PROTOZOA 



cipally in the endothelial cells of the spleen and liver, and in the 

 bone marrow. It is oval, two to four microns in diameter, finely 

 granular and occasionally vacuolated. It contains a large, round 

 nucleus and a smaller blepharoplast which is oval or rod shaped; a 

 third body, a slender short thread, may sometimes be recognized, 

 which is presumably the undeveloped flagellum. Stained specimens 



4^ ; 



FIG. 144. LEISHMANIA DONOVANI. (Army Med. School Collection, 

 Washington, D. C.) 



of blood, spleen and liver pulp, and bone marrow, usually show large 

 endothelial cells or leucocytes closely packed with parasites, one to 

 two hundred to a single cell. Multiplication in the body is by simple 

 division, and incompletely divided pairs of organisms are frequently 

 seen. Cultures have been obtained in citrated blood and on the 

 usual N. N. N. medium. When fully grown the cultural organisms are 

 typical herpetomonads (leptomonads) ; the cell body elongates and 

 the rudimentary whip develops into a true flagellum. Both dogs and 

 monkeys are susceptible to artificial inoculations. 



