INDEX OF SUBJECTS 



1185 



Spirillum Milleri in the normal mouth, 



219 



Spirochaeta anserina, 872 

 Spirochaeta calligyrum, 873 

 Spirochaeta Duttoni, 866 

 Spirochaeta gallinarum, 871 

 Spirochaete, genus, 42 



anaerobic tissues tubes for cultivation 



of, 167 

 Spirochaete Icterohaemorrhagiae, 885-888 



causative agent of Weil's disease, 886 



cultivation of, 886-887 

 Spirochaete macrodentium, 873 



microdentium, 873 

 Spirochaete of Obermeier, 861 

 Spirochaeta pallida, 849-861 



animal pathogenicity, 857 



cultivation of, 856-857 



demonstration of, 852-855 



Wassermann reaction with, 866 

 Spirochaeta pertenue, 870 

 Spirochaeta phagedenis, 872 

 Spirochaeta refringens, 846, 849 

 Spirochaetes, diseases 'caused by, 846 



Kat-bite fever, 873 



relapsing fever, 861 



syphilis, 849 



Vincent's angina, 867 



Yaws, 867 

 Spironema, 848 



Vincenti in the normal mouth, 219 

 Spontaneous generation, 4, 5, 6 

 Spores, 16 



conditions necessary for formation, 

 16 



destruction of by boiling, 83 



destruction of by fractional steriliza- 

 tion, 84 



destruction of by steam under pres- 

 sure, 85 



discovery of, 6 



germination of, 18 



loss of ability to form spores, 37 



position of, 17 



resistance to temperature changes, 36 



not related to arthrospores, 12, 16 



special stain for, 116 

 Sporothroichosis, 992 



Spirosoma, genus, 42 

 Sporotrichum (sporothrix), 993 



miuutissimum, 1009 

 Spine, 991 



Sputum, examination of, 215 

 collection of, 215 

 for pneumococcus typing, 215 

 for tuberculosis examination, 215 

 Sputum, Petroff's method of isolating 



tubercle bacilli from, 591 

 methods of concentrating tubercle ba- 

 cilli in, 589 

 antiformin, 589 

 Staining of bacteria, 113 

 acid-fast, 124 



Baumgarten 's, 125 

 Ehrlich's, 124 

 Gabbet's, 124 

 Hermann's, 126 

 Pappenheim ; s. 125 

 Ziehl-Neilson, 124 

 capsule stains, 116 

 Buerger, 117 

 Hiss, 116 

 Huntoon, 116 



Wadsworth's for smear and sec- 

 tion, 118 

 Welchi, 116 

 differential stains, Gram's method, 



121 



classification by, 123 

 discussion of chemical basis for, 



120 



Jensen's modification, 122 

 Paltauf's modification, 121 

 Sterling's modification, 122 

 discussion of chemical principles un- 

 derlying, 114 

 flagella stains, 118 

 van Ermengen's, 119 

 Loeffler's, 119 

 Smith's modification of Pitfield's 



method, 119 

 in tissues, 129 



Gram-Weigert, 130 



Loeffler's, 129 



Mallory's eosin methylene blue, 131 



method for staining acitomyces, 132 



