METHODS OF CULTURE AND OF EXAMINATION. Ill 



(d) Inoculation of the Anterior Chamber of the Eye. With 

 a cataract-needle a small incision is made at the junction of 

 the cornea with the sclerotic, the aqueous humor is per- 

 mitted to escape, and the infecting material is introduced. 

 The wound closes and heals rapidly. 



(i) Inoculation of the Cavities of the Body.-+- r T\ie needle 

 of the syringe is introduced into the selected cavity (pleural 

 or peritoneal), and the suspension of bacteria is injected. In 

 intraperitoneal inoculation the needle of the syringe, after 

 thorough cleansing of the abdominal wall, is introduced 

 subcutaneously in a horizontal direction ; then the syringe is 

 elevated, and the needle pushed on until the disappearance 

 .of resistance indicates that the extremity is free in the 

 abdominal cavity. . 



(/") Subdural Inoculation. A trephine-opening is made 

 to one side of the sagittal suture in order to avoid injury to 

 the longitudinal sinus, and, with the aid of a curved needle, 

 the fluid is introduced beneath the dura. 



(g) Inoculation by Inhalation. The bacterial mass is 

 minutely subdivided by means of a spray, which is intro- 

 duced through a tube into a closed space in which the 

 experimental animals are placed 



(/i) Inoculation through the Gastro-intestinal Tract. The 

 food of the animals is saturated with the bacterial fluid, or 

 this is introduced into the stomach by means of a tube, the 

 jaws of the animal being held apart with the aid of a hollow 

 wooden gag, through which an elastic so-called Nelaton 

 catheter is passed carefully into the stomach. 



For special purposes (introduction of bacteria into the 

 liver or into the portal vein or into a loop of intestine) celi- 

 otomy becomes necessary. After especially careful sterili- 

 zation of instruments, hands, and field of operation, the 

 cutaneous incision is made, the muscles are divided layer by 

 layer, and, finally, the peritoneum is divided upon a grooved 

 director. At the conclusion of the inoculation the wound 

 is closed by interrupted peritoneal, muscular, and cutaneous 

 sutures, and it is covered with iodoform-collodion. 



All of these various inoculations must naturally be car- 

 ried out with the most rigorous cleanliness. The skin at 

 the point of inoculation must be shaved and washed with 

 soap, solution of mercuric chlorid, alcohol, and ether. All 

 instruments employed in the inoculation are sterilized by 

 boiling in a one per cent, soda-solution. The disinfection 



