112 



CLINICAL BACTERIOLOGY. 



of the ordinary hypodermic syringe is more difficult, and 

 for this reason quite a number of sterilizable syringes 

 (Fig. 36) have been devised (Roux, Koch, Lewin). The 

 ordinary hypodermic syringe is conveniently and safely 

 disinfected by filling it with, and permitting it to remain 

 for from twelve to twenty-four hours in, a five per cent, 

 solution of carbolic acid, and then removing the carbolic 

 acid by repeated rinsing in sterilized water. In deal- 

 ing with especially infectious and conspicuously resistant 

 bacteria (anthrax and tetanus), however, the syringe of 

 Roux is employed, and it is boiled for ten minutes in a 

 one per cent, solution of soda. In inoculating the anterior 

 chamber of the eye the greatest care must be taken to 

 secure disinfection of the conjunctival sac. This is cleansed 



Fig. 36. i, Roux's bacteriologic syringe ; 2, Koch's syringe ; 3, Meyer's bacteriologic 



syringe. 



with a i : 3000 solution of mercuric chlorid, which is 

 removed by irrigation with sterilized water. Anesthesia is 

 effected by means of boiled cocain-solution. 



The inoculated animals are carefully observed, their tem- 

 perature is taken at regular intervals, the evacuations and 

 the occurrence of convulsions, etc., are noted, and, in short, 

 all manifestations of disease are looked for. Should an in- 

 oculated animal die, the autopsy is to be conducted with 

 every precaution. It is usually made as soon as possible 

 after death, in order to avoid the occurrence of putrefaction. 

 The animal, placed upon its back, is stretched upon a 

 board, with somewhat raised borders. The whole surface 

 of the animal is moistened with a solution of mercuric 

 chlorid, in order to prevent the generation of dust. The 



