MALARIA. 379 



state visible only as a bright spot within a red blood-cor- 

 puscle. It is actively motile, and extrudes numerous pseu- 

 dopods. In the first twenty-four hours (first phase of 

 Golgi) it develops gradually, and collects finely granular 

 pigment within itself, which continues in active vibratile 

 movement, with a preference for the outer layer of the 

 plasmic body. With increasing deposition of pigment the 

 activity of ameboid movement diminishes, without, however, 

 ceasing entirely. After twenty-four hours the parasite has 

 attained about half the size of the red blood-corpuscle, which 

 itself has lost in color and has undergone increase in size 

 often to a considerable degree. " The blood-corpuscles in- 

 fected with tertian parasites are frequently distended and 

 chlorotic." After forty -eight hours, when the parasite has 

 attained almost the size of the blood-corpuscle and is no 

 longer in motion and the pigment also is at rest, sporula- 

 tion takes place. Usually, the pigment moves again toward 

 the center, the plasma divides into from fifteen to twenty 

 round, highly refractive globules, which sometimes ar- 

 range themselves in two concentric rows (Golgi's sun- 

 flowers), but frequently lie together in the shape of berries, 

 resets, or grapes. The round globules are smaller than 

 the spores of the quartan parasites. The nucleolus is only 

 with difficulty to be distinguished in them. The spores 

 then become free, and after a time infect new blood-cor- 

 puscles, in turn themselves to repeat the same process of 

 development. By no means all parasites undergo sporula- 

 tion, just as is the case with the quartan parasite. A large 

 number of tertian parasites remain sterile. These barren 

 elements generally become as large as the parasites that 

 undergo propagation, or larger. In them, however, the 

 pigment remains actively motile. Laveran considers them 

 as hydropic and involved in degeneration. They may still 

 be visible in the blood for hours after the attack, and even 

 on the afebrile days. The act of sporulation corresponds 

 also in tertian fever with the febrile paroxysm. Golgi has 

 shown that as early as three hours before the chill the 

 temperature begins to rise, and that then the first spores 

 make their appearance in the blood. They are, however, 

 most numerous at the time . of the chill. In the mature 

 tertian parasites the formation of flagella can often be ob- 

 served. Kruse considers this process, likewise, as evidence 

 of degeneration. 



