408 



CLINICAL BACTERIOLOGY. 



tion appears. Guinea-pigs died after intraperitoneal injection, 

 with precisely the same symptom-complex as appeared after 

 introduction of true comma-bacilli. Similar vibrios have been 

 cultivated from Munich well-water by Weibel ; from Peene 

 water, by Loffler ; from the harbor of Groningen, by Fokker ; 

 and from the harbor of Altona, by Kiesling ; and, further, from 

 the Seine. In forming an opinion as to the identity of these 

 and the following vibrios with the true exciting agents of Asiatic 

 cholera, Pfeiffer's reaction (p. 187) and the agglutination-test 

 must be given preeminent importance. Both tests yield nega- 

 tive results. 



J7- Vibrio Metschnikoff, This was first cultivated in an epi- 

 demic among fowl, and subsequently from the water of the Spree. 



/c TKfc.1 fS r ,; , XT, '1 



;v% t; fe^'V v> ' 



^JK?-^'l^ 







Fig. 89. Spirillum berolinensis, from an agar-agar culture; X 1000 (Itzerott and 

 Niemann). 



It is somewhat thicker and shorter than the cholera-vibrio, and 

 sometimes almost coccus-shaped. In hanging drop it exhibits 

 active motility. The cultures resemble those of the cholera- 

 bacillus, except that liquefaction is more marked. The nitroso- 

 indol reaction appears within twenty-four hours. This vibrio, 

 in contradistinction from that of cholera, is pathogenic for both 

 pigeons and guinea-pigs. 



j. Vibrio Gindha. This organism was cultivated by von 

 Pasquale from well-water in Gindha near Massowah. It is a 

 rather long, slightly curved rod, actively motile (with a single 

 terminal flagellum). It is but slightly pathogenic, and it does 

 not yield the nitroso-indol reaction. 



jp. Vibrio Lissabon. This has been cultivated in the course 



