53 



with like precautions. At the same time of day on the 10th, 

 it was fed six worms. On the 11th, 12th, 1.3th, 14th and 15th, 

 it was fed ten worms daily. At the same time from the first 

 feeding, a little less than seven days, it had the gapes. It was 

 killed in eight days and twelve gape worms found, all in the 

 trachea. 



Exp. 3. November 13th, three chicks, two days old, were fed 

 earthworms from my garden, eight, nine, and ten days, respec- 

 tively. No symptoms of the gapes were produced, but to deter- 

 mine positively, the one fed nine days was killed, and no gape 

 worms found. This experiment shows that all earthworms do 

 not contain the embryos of Syngamus. To confirm this, earth- 

 worms from the same place have been repeatedly examined with 

 the microscope, and none of the embryos found. 



Exp. 4. Two mature Syngami were broken in pieces, so as to 

 free the eggs. They were then placed on the surface of a dish 

 filled with dirt, well moistened with water. After two weeks, 

 some earthworms were placed in this dish and allowed to remain 

 ten days. Three of these were fed to a chick, which was care- 

 fully watched for two weeks. No symptoms of gapes were 

 discovered. Evidently, the embryos had not obtained access to 

 the earthworms in sufficient numbers to produce the disease in 

 chicks. 



Exps. 5 and 6. On December 13th, two chicks, four weeks and 

 four days old, were each fed six earthworms from the infected 

 spot, with the same precautions as before. On the 14th, 15th 

 and 16th, the feeding of six Worms was repeated, making twenty- 

 four to each chick. On December 20th, about seven days, as 

 before, they had the first symptoms of the gapes. One was now 

 killed, and twenty-two Syngami were found. On December 

 24th, eleven days from the first feeding, the other was killed, 

 and sixteen found. All of them were united, and in the trachea. 

 None were found in the lungs, but it is probable they were 

 there, and want of experience in the search prevented their 

 discovery. 



Exps. 7, 8 and 9. On April 21st, 1884, fed three chicks, two 



