748 HISTOLOGIC TECHNIC 



This method gives an excellent stain for the finer nuclear structure, 

 mitosis, etc. 



Muchematein (Mayer) : 



Hematein (Griibler's) 0.2 grm. 



Glycerin 40 c.c. 



Aluminium chlorid 0.1 grm. 



Distilled water 60 c.c. 



Mix the ingredients in the order given, rubbing the hematein with 

 the glycerin in a mortar. One or two drops of nitric acid added to the 

 final mixture will sharpen its properties as a nuclear stain. 



This dye is used as a specific stain for mucinous tissues. It is used 

 in the same manner as hematein, and stains rapidly (three to ten 

 minutes) . 



Mucicarmin (Mayer) : 



Carmin 1 grm. 



Aluminium chlorid 0.5 grm. 



Distilled water 2 c.c. 



50 per cent, alcohol 100 c.c. 



Mix in the order given ; heat over a small flame till the fluid darkens 

 (two minutes) ; after twenty-four hours, filter. For use, dilute with 

 five to ten volumes of 50 per cent, alcohol. Like muchematein, mucicar- 

 min is a specific stain for mucus-containing cells. It also stains 

 rapidly. 



Weigert-Pal Stain for Medullated Nerve Fibers. The tissues 

 must have been previously fixed in Mutter's -fluid or in 10 per cent, 

 formalin, washed in water, hardened in alcohol, and sectioned. 



I. Stain: 



Hematoxylin 1 grm. 



Absolute alcohol 10 c.c. 



Distilled water 90 c.c. 



Saturated aqueous solution of lithium carbonate 



(1 to 80) 1 c.c. 



II. Differentiating Solution: 



Potassium permanganate 0.25 grm. 



Distilled water 100 c.c. 



