754 HISTOLOGIG TECHNIG 



only to tissues which have been fixed in Zenker's solution and de- 

 hydrated with alcohol. Somewhat inferior results are obtained after 

 fixation with mercuric chlorid. 



It stains collagenous fibers and reticulum, blue; nuclei, neuroglia 

 and cytoplasm, red; and hemoglobin (red blood corpuscles), yellow. 

 Elastic fibers remain unstained. 

 I. Stain: 



Acid fuchsin 0.1 grm. 



Distilled water > 100 c.c. 



II. Fixative: 



Phosphomolybdic acid 1 grm. 



Distilled water 100 c.c. 



III. Counter-Stain: 



Anilin blue (soluble in water) 0.5 grm. 



Orange G- (Griibler's) 2 grm. 



Oxalic acid 2 grm. 



Distilled water 100 c.c. 



1. Stain in the fuchsin solution, three to twenty minutes. The 

 sections should become a bright red. 



2. Wash in water. 



3. Fix in the phosphomolybdic acid solution, one minute. This 

 'fixes' the fuchsin and prevents decolorization. 



4. Wash well in water. 



5. Counter-stain in the anilin blue solution (No. Ill), five to 

 twenty minutes. The section should become decidedly blue. 



6. Wash in water. 



7. Dehydrate, clear, and mount. 



Eosinate of Methylene Blue (Basting's Method for Blood). For 

 the somewhat complicated method of preparing the stain the reader is re- 

 ferred to the original article, Johns Hop. Hosp. Bull., 1904, vol. xv, p. 

 122. The stain is applicable to smears of blood, marrow, splenic cells, 

 etc. When used with smears which contain traces of fat, a preparatory 

 treatment with a 2 per cent, aqueous solution of sodium metaphosphate, 

 which probably serves as a mordant, improves the staining properties. 

 Otherwise the stain is applied without previous fixation. 



1. Stain for one minute. 



2. Dilute the stain with several volumes of distilled water, and 



