HISTOLOGIC TECHNIC 759 



3. Wash in water. 



4. Place in a 5 per cent, aqueous solution of oxalic acid for "five 

 to ten minutes. 



5. Wash thoroughly in water. 



6. Place in phosphotungstic acid hematoxylin solution for twelve 

 to twenty-four hours. 



7. Dip for a few seconds in 95 per. cent, alcohol. 



8. Clear in carbol-xylol and xylol and mount in xylol-damar. 



Vance's Method for Staining Bile Canaliculi (Anat. Anz., Vol. 44, 

 No. 17, pp. 412-413). 



1. Fix tissues in Helly's fluid; or in a mixture of equal parts of 

 10 per cent, formalin and 5 per cent, mercuric chlorid. 



2. Embed in colloidin. 



3. Place sections in a dilute solution of iodin in 95 per cent, alco- 

 hol for five to fifteen minutes. 



4. Remove excess iodin by washing in several changes of 95 per 

 cent, alcohol. 



5. Stain in Mallory's phosphotungstic acid hematoxylin for twenty- 

 four hours. 



6. Transfer directly to 95 per cent, alcohol. 



7. Clear in carbol-xylol or in oil of origanum, and mount in balsam. 



Jenner's Blood Stain. This solution acts as a fixative as well as a 

 stain. Blood smears are immersed in the stain for two to five minutes, 

 washed in water, dried and mounted in balsam. 



Water soluble eosin (Grublers), 1 per cent, aque- 

 ous solution 100 c.c. 



Methyl ene blue (Griibler's), 1 per cent, aqueous 



solution 100 c.c. 



Mix the above solutions ; after twenty- four hours, filter. Dry the 

 filtrate at 65 C., wash the dry filtrate, and again dry and powder. Dis- 

 solve 0.5 gram of the powder in 100 c.c. pure methyl alcohol. The 

 result constitutes the stock solution. 



Zimmermann's Technic, for demonstrating the intercalated disks 

 of cardiac muscle. 



1. Small pieces of tissue are fixed for twenty-four hours in a solu- 

 tion of 90 parts absolute alcohol and 10 parts 25 per cent, nitric acid. 



2. Wash in several changes of 95 per cent, alcohol, or until it re- 

 mains neutral to litmus paper. 



