DIRECTIONS FOR LABORATORY WORK 803 



(3) Study a stained longitudinal (sagittal) or radial section of the 

 mammalian kidney. Make l.p. sketch (289) (diagrammatic) far show 

 the finer topographic relationship of the several divisions and their 

 larger constituents. Note cortex corticis, pars radiata, pars convoluta, 

 renal corpuscle, renal column, and boundary and papillary zones of the 

 medulla. 



Sketch h.p. (2$0) a renal corpuscle. Note: glomerulus; capsule 

 (visceral and parietal layers) ; arterial pole (with afferent and efferent 

 arterioles) ; uriniferous pole (neck). 



Sketch h.p. (291) portions of the several segments of a renal (urinif- 

 erous) tubule: proximal convoluted tubule; descending limb of Henle's 

 loop; ascending limb of Henle's loop; distal convoluted tubule; arched 

 collecting tubule; straight collecting tubule; papillary duct. 



Note the difference in staining reaction of the two portions of the 

 tubule, divided at the point where the distal convoluted joins the arched 

 collecting tubule. Significance with respect of function? of origin? 



(4) From a demonstration slide prepared according to Huber's tech- 

 nic for isolating the renal tubules (Anat. Rec., vol. 5, 1911), sketch a 

 complete tubule (292). 



(5) Study a stained coronal (tangential) section of the kidney 

 through cortex. Identify the several constituents of the pars radiata 

 and the pars convoluta. Make a similar study of a similar section 

 through the medulla. 



(6) Study a longitudinal section of the kidney stained with Mai- 

 lory's connective tissue stain. Note the types and distribution of the 

 connective tissue. 



(7) From a demonstration slide of the kidney prepared by Meve's 

 mitochondrial technic, sketch (293) a portion of the proximal convo- 

 luted tubule. Shape of the mitochondria? Function? 



(8) Study a radial section of an injected cat's kidney. Sketch (294) 

 the blood supply. Note interlobar arteries and veins; arciform (arcu- 

 ate) vessels; interlobular vessels; afferent and efferent glomerular ar- 

 terioles; the glomerular rete mirabile; the capillary supply of the con- 

 voluted tubules; the stellate veins; the arteriolae and venulae rectse (verae 

 and spurise) of the medulla. 



(B) THE URINARY BLADDER (Vesica urimria). 



Study l.p. a stained section of the urinary bladder. Make l.p. sketch 

 (295) of segment of complete wall; h.p. sketch (296) of tunica mucosa. 

 How does the mucosa vary with the degree of distension ? How does the 



