818 DIRECTIONS FOR LABORATORY WORK 



(B) THE CEREBELLUM. 



(1) Study l.p. a stained parasagittal section of a hemisphere of the 

 human cerebellum. Note the arrangement into lobules composed of 

 folia. Note also that each folium comprises a central medulla and a 

 peripheral cortex. In the cortex identify the superficial molecular layer, 

 the deeper nuclear or granular layer, and between the two the inter- 

 mediate layer of Purkinje cells (commonly classified as the innermost 

 stratum of the molecular layer). Sketch (408). Make h.p. sketch 

 (409) of a Purkinje cell. 



i (2) From a specially prepared demonstration slide of the cerebellum 

 (Golgi technic) make a diagram (410) showing the location and inter- 

 relations of the constituent cells and fibers: In the molecular layer, the 

 large ('basket') and small cortical cells; the Purkinje cells; and in the 

 nuclear layer, the granule cells, eosin bodies, large stelate cells, and the 

 solitary cells. In the medulla note the axons of the Purkinje cells, the 

 mossy fibers and the climbing fibers. What is the significance of the 

 eosin bodies? Eelation between granule cells and mossy fibers? How 

 does a sagittal section differ in appearance from a frontal section, with 

 respect to the Purkinje cells and the large cortical (basket) cells? Note 

 also the types and disposition of the neuroglia cells. 



(C) THE CEREBRAL CORTEX (Pallium). 



(1) Study l.p. a stained (hematoxylin and eosin) vertical section of 

 the human cerebral cortex in the motor area (precentral gyrus). Note 

 the arrangement of the cells into five tangential strata : molecular layer, 

 outer polymorphous cell layer, small pyramidal cell layer, large pyramidal 

 cell layer, and inner polymorphous cell layer. Sketch (411). Compare 

 with similar sections from the parietal lobe (a sensory area), the visual 

 area of the occipital lobe, the auditory area of the temporal lobe, and 

 the olfactory area of the hippocampal gyrus. Note also the cerebral 

 meninges (dura mater, arachnoidea, and pia mater) and the enclosed 

 lymph spaces. Describe the granulationes arachnoidales (Pacchionian 

 bodies; arachnoidal villi). 



(2) From a Golgi preparation of the motor cortex, sketch (412) a 

 segment showing the character and arrangement of the neurons and the 

 neuroglia elements. Identify a cell of Betz; a cell of Martinotti. Sig- 

 nificance of these cells? Distribution of the 'solitary cells' of Meynert? 



(3) From a Weigert-Pal preparation of the motor cortex sketch 

 (413) the arrangement of the nerve fibers. Note the bands of radial 



