4 2 ZOOLOGY. 



The gelatine should be soaked in cold water and then 

 slightly heated until dissolved. The glycerine carmine may be 

 prepared as follows : thoroughly pulverize and mix 3 grams 

 of carmine with a little water, with enough ammonia added to 

 dissolve the carmine. Add 50 grams of glycerine. Mix and 

 filter. Add gradually to this mixture enough acidulated gly- 

 cerine (glycerine and acetic acid in the ratio of 10 to i) to 

 give a slight acid reaction to the carmine glycerine mass. 



5. Materials for Study. The types of animals needed for 

 this course, with the exception of the marine representatives, 

 may be secured in almost any locality, if sought at the proper 

 time. The teacher should become entirely familiar with the 

 common animals to be found within a reasonable distance 

 from his school. It is especially necessary to know the life 

 most abundant in the various ponds, lakes and streams. A 

 close watch should be kept on the material gathered from each 

 place, and a record kept of the various localities in which each 

 useful type has been found and of the best time for collection. 

 In time the laboratory will come to have an interesting set of 

 facts, valuable not merely in assisting in the finding of needed 

 material, but as indicating local distribution (see also 455; 

 IV, 4). The students should be encouraged to make excur- 

 sions, both with and without the teacher, to collect material 

 and extend the knowledge of the locality. 



If for any reason living materials cannot be secured in the 

 locality of the school, preserved specimens of marine, fresh 

 water, and terrestrial species may be secured of dealers. The 

 principal are: 



Supply department, Marine Biological Laboratory, Wood's 

 Holl, Mass. (Preserved materials.) 



Mr. F. W. Wamsley, Academy Natural Science, Philadel- 

 phia, Pa. (Preserved materials.) 



Henry M. Stephens, Carlisle, Penna. (Preserved and living 

 material.) 



Messrs. H. H. and C. S. Brimley, Raleigh, N. C. (Pre- 

 served and living: frogs, turtles, alligators, etc., in the winter.) 



