240 



The Fermentation of Cacao 



deeper layers of the beans in a sweating-box ; 

 when it is withdrawn, the small amount of 

 matter adherent to the loop is shaken up in a 

 saline solution, and various dilutions of this are 

 made. These dilutions are now either shaken 

 up in the molten agar media at 45 C. and 



FlG. 4. Sloped agar tube showing yeast growing on 

 the sloped surface. 



poured on to Petri's plates, where they are 

 set into solid media, or else the dilutions are 

 poured over the surface of media which have 

 already set on these plates. These plates 

 are now incubated at between 37 and 40 C., 



