THE VACCINE OF SPLENIC FEVER. 243 



It required all the efforts of his family to prevent him 

 from inoculating himself with the most virulent virus. 



An extraordinary movement was everywhere pro- 

 duced in favour of vaccination. A great number of 

 agricultural societies wished to repeat the celebrated 

 experiment of Pouilly-le-Fort. The breeders of cattle 

 overwhelmed Pasteur with applications for vaccine. 

 Pasteur was obliged to start a small manufactory for 

 the preparation of these vaccines in the Eue Vauquelin, 

 a few paces from his laboratory. At the end of the 

 year 1881, he had already vaccinated 33,946 animals. 

 This number was composed of 32,550 sheep, 1,254 

 oxen, 142 horses. In 1882, the number of animals 

 vaccinated amounted to 399,102, which included 

 47,000 oxen and 2,000 horses. In 1883, 100,000 

 animals were added to the total of 1882. 



From the commencement of the practical appli- 

 cation of this new system, the results were topical. 

 Among flocks where half had been vaccinated and 

 the other half not vaccinated all the animals con- 

 tinuing to live together the mortality from splenic 

 fever in 1881 was ten times less in the vaccinated 

 sheep than in the non-vaccinated, being 1 in 740 

 as against 1 in 78 ; and in cows and oxen fourteen 

 times less, being 1 in 1,254 against 1 in 88. In 1882 

 also, the mortality was ten times greater among the 

 non- vaccinated than among the vaccinated animals. 



In 1883 it was proved that the duration of the 



