PROTEIN. 179 



Mulder confirmed these views by analyzing several of the salts 

 of xantho-proteic acid.* 



He dissolved pure xantho-proteic acid in ammonia, and the 

 beautiful red liquid formed was evaporated on the water bath 

 till all the uncombined ammonia was driven off. The residue 

 was divided into two portions, the first of which was dried, and 

 the other again dissolved in water, and a current of chlorine gas 

 passed through it. The dried portion when heated to 212 gave 

 out ammonia. It lost its red colour and became orange yellow. 

 It was analyzed after being dried at 284. 326 parts gave 618 

 of carbonic acid and 198 of water. The azote per cent, was es- 

 timated at 14 *3 7. Hence its constituents are, 



Carbon, . 5170 or 34 atoms = 25*5 

 Hydrogen, 675 or 25 atoms 8-125 



Azote, . 14-37 or 4 atoms = 7*000 

 Oxygen, . 27-18 or 13 atoms - 13-000 



100- 53-625 



It is obviously composed of one atom xantho-proteic acid, and 

 one atom water. The ammonia had escaped and the hydrat- 

 ed acid remained. 



When a current of chlorine was passed through the aminoni- 

 acal solution of xantho-proteic acid it lost its red colour and 

 white flocks with a shade of yellow precipitated. When 

 washed and dried at 212, these flocks became lemon yellow. 

 This substance being analyzed was found to be a compound of two 

 atoms of hydrated xantho-proteic acid, and one atom of chlorous 

 acid. The analysis gave, 



Carbon, . 49-61 or 68 atoms = 51- 

 Hydrogen, 6-22 or 50 atoms = 6 '25 



Azote, . 12-89 or 8 atoms = 14- 



Oxygen, 23-29 or 26 atoms = 26- 



Chlorous acid, 7-36 or 1 atom = 7 -5 



99-37 104-75 



When the lemon yellow powder is dissolved in ammonia, azo- 

 tic gas is evolved. If we evaporate to dryness and dissolve off 

 the salamoniac by alcohol, we have the xantho-proteic acid in a 

 Ltate of purity.f 



* Ann. der Pharm. xxviii 78. t Ibid, xxxvi. 81. 



