STUDY NUMBER ; 6.2 



ORGANIZATION : Environmental Protection Agency (EPA) 



TITLE OF STUDY ; Chronic Bioassays of Champion Internation- 

 al's Effluent 



CONTACTS ; Dick Montgomery - EPA - 449-5486 

 Loren Bahls, DHES, WQB - 444-2406 



OBJECTIVE ; Determine the toxicity of Champion Internation- 

 al's effluent to juvenile rainbow trout and Ceriodaphnia 

 (water "fleas") . 



DURATION ; 1985 - ? 



CURRENT STATUS ; From May 13 to June 12, 1985, thirty-day 

 flow-through bioassays were conducted using recently emerged 

 (button-up stage) rainbow trout. Dilutions of Champion waste 

 water that were tested ranged from 2.0% (1;50 dilution) to 

 0.2% (1;500 dilution), plus controls (0.0% effluent). Two 

 separate dilution sources were used for the trout tests-- 

 river water and local well water. Mortality to trout in both 

 tests was very low (10% or less). There were significant 

 differences in "condition coefficients" --determined by 

 dividing wet weight by length--but there was not a clear 

 dose-response relationship with the various wastewater 

 dilutions. 



A seven-day Ceriodaphnia test was conducted using the 

 same dilutions of Champion wastewater as with the trout 

 bioassay, plus the addition of a 4.0% (1:25) dilution. Only 

 river water was used in these bioassays; daphnid survival in 



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